FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30783.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30783.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11093977
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC458180.4129988731723529No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG334380.30140679036922463No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA195290.17603245436690557No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC146820.13234208075246595No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA141080.12716810211522883No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT130370.1175142151457498No Hit
CGGCGAGCGAACGCGAAGGAGCCTGTTAGTGATAATGACAGAGACAGAGG116410.10493081065518706No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGATAGTGCGAGC112760.1016407371315084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA231900.040.3361241
GGTTAGA93450.034.6871534
GAGCAAT79000.034.612826
AACGTAT300950.033.523976
GCAATTA92700.033.412138
ACGAGCA77750.033.0174334
GGGAACG303500.032.1584783
TCACGAG78500.031.9593492
TGTCCTA50600.031.2463515
ACGTATT332850.030.6404237
CAATTAG101800.030.568039
AGCAATT93800.030.545857
GAACGTA346800.029.8427075
GGAACGT338150.029.3156534
GTATTCA346250.029.1403889
CGAGCAA97350.028.235575
CCATTGT223600.027.955677145
CGTATTC394450.025.597848
CGGGAAC389650.024.5263082
TATTCAC319950.024.3716169