FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56530503
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10928731.9332447829095027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACGT189750.023.5720214
CCGGGAA183450.022.8899021
CGGGAAC194150.022.4015712
GAACGTA225700.022.0361275
GTATTCA228650.021.6997039
GATCCTG71850.019.7775295
TATTCAC224550.018.4508939
GGGAACG263750.017.7821143
CGATCTA33600.017.6916374
CCATTGT149200.017.510504145
CTGGCTC86400.016.3476499
TTTGATC109500.015.9539242
TGATCCT92100.015.2698784
TGTCCTA52200.015.1253897
TGGGCTA57100.015.1069052
CGAGGGA96350.014.9603568
GAAACTA83400.014.689744
TCCTGGC92700.014.5339547
TTGATCC95750.014.3085163
TGCCGTG77700.014.236249145
Produced by FastQC (version 0.11.5)