FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_9424.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_9424.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14601642
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC215800.14779159768469874No Hit
CTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTCGCTACAAAATATA184980.12668438248246328No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG156450.10714548404898573No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC150690.10320072222014484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATTA46700.053.86241145
TACTACT41250.035.8476036
TTTGATC105600.035.556442
CCGGGAA267450.034.898521
ATTAGAA56500.033.997846
CTCGGTT143850.033.274011
CGATCTA26400.032.3986934
AACGTAT352450.030.5204036
CAATTAG63400.029.9545619
TCAGATT62100.029.7646662
CTATTAG45150.028.7373168
GTATTCA383450.028.4686689
GGGAACG359350.027.6547183
CGGGAAC343500.027.4749342
ACGTATT402200.027.1595217
TGGGCTA61950.027.0285722
CGTATTC403400.026.9529488
AGCAATT50200.026.5683787
GCAATTA73700.025.866598
CCTGATT83150.025.6293452
Produced by FastQC (version 0.11.5)