FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_9424.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_9424.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14601642
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC215800.14779159768469874No Hit
CTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTCGCTACAAAATATA184980.12668438248246328No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG156450.10714548404898573No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC150690.10320072222014484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATTA46750.053.64985145
TTTGATC105300.035.451292
TACTACT41250.034.9690486
CCGGGAA266900.034.8075871
ATTAGAA55850.033.874436
CTCGGTT143850.033.576681
CGATCTA25900.032.464484
AACGTAT351000.030.5432856
CAATTAG63750.029.903869
TCAGATT61900.029.860892
CTATTAG44850.028.7679738
GTATTCA381750.028.4625849
GGGAACG358400.027.6471733
CGGGAAC342400.027.4785692
TGGGCTA61800.027.4460962
ACGTATT399800.027.2138257
CGTATTC401100.026.981058
AGCAATT49950.026.8465187
GCAATTA73300.026.0077938
CCTGATT83450.025.9715612
Produced by FastQC (version 0.11.5)