FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_9.21.2018.neg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_9.21.2018.neg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10362677
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1691861.6326476257052112TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA507610.48984446779533897TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT174690.16857613143785144TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC31350.035.376259
AACAGAC42700.027.1613965
AGACATA57000.024.6707698
TTGTACT59150.024.3986531
TCTAGTT69650.023.6243867
CGTCTTG121450.023.218554145
AGTCACA59000.022.484444145
GAGTCTA16150.022.441794
CTGGCTC41950.022.1174939
TAGCATA32100.021.6781398
TGTACTC67400.021.6169322
TCTCGTT139600.021.238358145
GTAGCAT34000.020.8931057
GCTGAAT41000.020.6951941
CTCCAAC65800.020.3898181
TGCCGTG175000.019.800352145
AAATCTG88800.019.428846145
TCCAACA61400.019.361252
CCAGTAG41100.019.2240414
CGAAATG256050.019.110062145
Produced by FastQC (version 0.11.5)