FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_9.21.2018.neg_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_9.21.2018.neg_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10362677
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1691861.6326476257052112TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA507610.48984446779533897TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT174690.16857613143785144TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC31300.035.895969
AACAGAC42450.027.4921365
AGACATA57150.024.8597038
TTGTACT59450.024.275431
TCTAGTT70350.023.492377
CGTCTTG121600.023.190037145
AGTCACA59200.023.143312145
CTGGCTC42100.022.0387049
TAGCATA32000.021.5193798
TGTACTC68050.021.4104672
GAGTCTA16400.021.2157154
TCTCGTT140150.021.103401145
GCTGAAT41150.020.9721431
GTAGCAT33750.020.8331097
CTCCAAC65850.020.5945131
TCCAACA61700.019.6195722
TGCCGTG175050.019.339275145
AAATCTG89250.019.330994145
CCAGTAG40650.019.0802254
CGGATGT35950.018.962461
Produced by FastQC (version 0.11.5)