FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_8230.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_8230.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19135582
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC402750.21047177974518883No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT395460.20666212294980107No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC301900.1577689144756611No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC289810.1514508416833102No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC272970.14265048222729781No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA271330.14179344009500208No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG270120.14116111022910097No Hit
CCCAAATCGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGT244490.12776721397864982No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT235800.12322593585081447No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT225990.11809936065702105No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG219070.11448306092806584No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA213770.1117133515980857No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC203260.10622096573806848No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA198090.10351919267467277No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT194980.10189394814330707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGA147200.045.6972163
CTCCCTG158400.042.3289262
TCGACAT93950.040.5819447
AATCGAC129100.035.8216135
CCCTGAG198050.034.1838954
CCCAAAT106100.033.971191
ATAGATG122200.033.33757145
GAGTCTA61300.032.517932
AAATCGA148000.031.8347324
GAAACTA65100.031.287794
TCAGATT106400.031.2014332
CCTGAGC225500.030.6013855
CCAAATC119300.030.5009922
CTGAGCT230400.030.2651026
AGTCTAA67900.029.1435493
TGAGCTC242650.029.0654097
GATCATG75350.029.051895
GATCCTG63600.028.9485825
ATCGACA136600.028.442236
TTGAACG117400.028.1540347
Produced by FastQC (version 0.11.5)