FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_8230.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_8230.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19135582
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC402750.21047177974518883No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT395460.20666212294980107No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC301900.1577689144756611No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC289810.1514508416833102No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC272970.14265048222729781No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA271330.14179344009500208No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG270120.14116111022910097No Hit
CCCAAATCGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGT244490.12776721397864982No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT235800.12322593585081447No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT225990.11809936065702105No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG219070.11448306092806584No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA213770.1117133515980857No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC203260.10622096573806848No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA198090.10351919267467277No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT194980.10189394814330707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGA146750.045.3924523
CTCCCTG158200.042.10722
TCGACAT94200.040.0892037
AATCGAC128600.035.6224255
CCCTGAG198150.033.800584
CCCAAAT105750.033.5348241
GAGTCTA61100.032.9800222
ATAGATG122550.032.414013145
AAATCGA146800.031.7984564
GAAACTA65050.031.5344584
TCAGATT106650.031.196022
CCTGAGC225900.030.2260955
CCAAATC118800.030.0189862
CTGAGCT230600.029.9242926
AGTCTAA67250.029.7483633
GATCCTG63150.029.0398625
GATCATG74950.029.013295
TGAGCTC243800.028.660517
TTGAACG117450.028.203557
ATCGACA136450.028.0483066
Produced by FastQC (version 0.11.5)