FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_8230.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_8230.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886163
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC679900.4567328733401616No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG519310.3488541674573898No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT383510.25762851044960344No Hit
CGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGA247400.16619460636028235No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT237180.15932917031742835No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC234950.15783113485993672No Hit
CTTTACTATAGCTTGACACTGAACATTGAATTTTGATGTGTAGGATAGGT232110.1559233228871671No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC231920.1557956875791297No Hit
ATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATA231650.15561431108876075No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC215640.14485935697466165No Hit
TGAAGATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTA203820.13691909728517684No Hit
CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCG170340.11442841247942803No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGC162070.10887291775590527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA352200.048.46841
GGAACGT479900.037.715364
GAACGTA488250.037.5601435
GGGAACG481000.037.4634633
AACGTAT495700.036.9520236
ACGTATT507200.036.2855647
CGTATTC528100.034.7671788
CGGGAAC509550.033.9846422
GTATTCA553900.033.3050279
ATAGATG155000.030.961704145
CCATTGT329650.030.699474145
CATTGTA257350.029.943851145
TATTCAC535550.028.2607579
CGTCTTG58850.024.513493145
CCCGGGA254800.022.8723092
CCGAGTC81350.022.4539857
TTGAACG89400.021.242927
GTGGATG119900.020.6757032
AGATGCG171950.020.0235884
TGGATGC121950.019.9714373
Produced by FastQC (version 0.11.5)