FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_8230.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_8230.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886163
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC679900.4567328733401616No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG519310.3488541674573898No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT383510.25762851044960344No Hit
CGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGA247400.16619460636028235No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT237180.15932917031742835No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC234950.15783113485993672No Hit
CTTTACTATAGCTTGACACTGAACATTGAATTTTGATGTGTAGGATAGGT232110.1559233228871671No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC231920.1557956875791297No Hit
ATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATA231650.15561431108876075No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC215640.14485935697466165No Hit
TGAAGATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTA203820.13691909728517684No Hit
CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCG170340.11442841247942803No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGC162070.10887291775590527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA352400.048.647071
GGAACGT480250.037.853824
GAACGTA488100.037.7052655
GGGAACG480600.037.660483
AACGTAT495900.037.0832186
ACGTATT507300.036.421237
CGTATTC527850.034.92098
CGGGAAC509350.034.1686942
GTATTCA554800.033.3815849
CCATTGT328950.030.764742145
ATAGATG155250.030.678314145
CATTGTA257850.029.548353145
TATTCAC536250.028.156239
CGTCTTG58650.024.72064145
CCCGGGA254700.022.7674062
CCGAGTC81350.022.6321477
TTGAACG90100.021.0778397
GTGGATG119900.020.5547522
AGATGCG173100.020.0998954
TGGATGC121200.019.9155673
Produced by FastQC (version 0.11.5)