FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_7651.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_7651.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14353270
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAAAG714040.4974754881640212No Hit
GTGCTTTTTAACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTA536820.3740053660246062No Hit
CTCAAACTTCCATCGGCTTGAGCCGATAGTCCCTCTAAGAAGCCGGCGAC523330.3646068108521612No Hit
GGAGTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAAT385450.2685450771845022No Hit
CCCGGATCAGCCCGCGAAGGACTTTAATGCAAGAAAATGGTCGATGAAGA375020.2612784403832715No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT352830.24581854866521707TruSeq Adapter, Index 27 (97% over 39bp)
CGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGC325580.2268333278758081No Hit
CTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTG316120.22024249526414538No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAA306640.21363772854548127No Hit
CCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTACCACCA298280.2078132718188956No Hit
GTTAGTTTTACCCTACTGATGACCGTCGTCCCAATGGTAATTCCGCTTAG297040.20694935718480875No Hit
GCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCC265550.18501010571110277No Hit
CTCGAACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAA263460.18355399152945637No Hit
CCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCA260760.1816728870842672No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT245830.1712710762077213No Hit
AAGGAGTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTA231680.16141269550423004No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT228430.15914840311650236No Hit
GGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA221580.1543759714685225No Hit
CCGGGGCCGAAGCCTATTCGTATCCTCGTACGACGTGCTGGAGAAAATCG206740.14403686407348293No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA203330.141661098829744No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCG197580.13765504306684123No Hit
CCGGCACCTTAACCTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT195680.1363313029017081No Hit
AGACAGGTTAGTTTTACCCTACTGATGACCGTCGTCCCAATGGTAATTCC194860.13576000451465065No Hit
CCCGACTATCCCTATTAATCATTACGGCGGTCCTAGAAACCAACAAAATA193600.1348821557735624No Hit
GCTCGAACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCA184920.12883475333495434No Hit
CCCCGTTACCCGTTGATACCATGGTAGGCCACTATCCTACCATCGAAAGT183990.1281868173593892No Hit
GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT182770.1273368368323037No Hit
CTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCCT177400.12359552910242753No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA169870.11834933781640002TruSeq Adapter, Index 27 (97% over 39bp)
GTTTTACCCTACTGATGACCGTCGTCCCAATGGTAATTCCGCTTAGTACG165060.11499818508256307No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATT164920.11490064633355326No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAAC163990.11425271035798812No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT162920.11350723563341314No Hit
CGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAG160640.11191874743525343No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG160560.11186301100724783No Hit
CCAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCGAACGGGTCAAAAT159010.11078311771463925No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAAC155830.10856759470141647No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACATGC155780.10853275943391297No Hit
GCACGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACC152520.10626149999268458No Hit
CGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG152160.10601068606665937No Hit
GACAGGTTAGTTTTACCCTACTGATGACCGTCGTCCCAATGGTAATTCCG149600.10422712037048004No Hit
GCGATGGCCAGAAAATGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTC148700.10360008555541698No Hit
GCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACA147350.1026595333328224No Hit
GTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTGCTAAA146240.10188619039424465No Hit
CCCGTTGATACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAGGG143710.10012352585856742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTC38050.068.771312
TTGGTTC86550.060.7191472
TCTCTTG44600.059.8091625
GATCTCT47700.055.0100333
CTCGAAC90850.054.3590851
GAGTCTA110950.054.3564072
TGGCATC94850.053.8773889
AGTCTAA113750.052.954313
ATCTCTT51250.051.199584
CTAACAT237500.050.503541
TAACATC238500.050.1476172
TCGAACA86200.049.4452742
GTAGGGA64650.048.4361842
CATCTAT235600.048.3650865
TGTTACG48900.047.582573
ATCTATG240350.047.439586
TCTATGC241300.047.162537
TAACTCT187950.046.89719
AGGAGTC82200.046.8249632
TTAACTC188600.046.4662748
Produced by FastQC (version 0.11.5)