FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_6852.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_6852.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCAAGTTGATCCTTTCAAAAAGACTGATATAATGAAAAATTTGAAATT12.0No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG12.0No Hit
AGGGAATGGGTGGCCCTCACCTCACTTTTGTCCCTCTCAGTTACAGCTCA12.0No Hit
GTTGGAAACTGCATGACTAGAGTACTGGAGAGGTAAGCGGAACTACAAGT12.0No Hit
GAAGCCGCGAGGTGGAGTGAATCTCAGAAAGTACGTCTAAGTCCGGATTG12.0No Hit
CTAGCCAAACTGGTAAAAACCAATTCATCGCGAATTTCTGGATTCGAATC12.0No Hit
GTTTTTTGTGCTTGGGTGGGGCTAGAATTTTTCGAATACCCATCTATATG12.0No Hit
TGCAAGTCGAACGATGAAGCCTAGCTTGCTAGGTGGATTAGTGGCGAACG12.0No Hit
GCGTGGTTGTGCTCCGCGGTGAGAGAGCGGTAGAGGAGATTCTCCTCGAA12.0No Hit
GGGAGCCTGTGGGGCTGCGGAGGGAGCAGGCTGGGGGCCAACGAGTGCAG12.0No Hit
AGCAGGAGTGTTTACCAAAGACATTGATAAAGCCATAACAATCTCCTCTG12.0No Hit
GCGATACTAGGGTGAGAAACCCTAGCGCCGAATGACCAAGGGTTCCAGGG12.0No Hit
GAGCTAGACTGCGAGACTGACCGGACGAGCAGGCACGAAAGTGGGAGTTA12.0No Hit
CAGAATCAATATGGTGGAACCCGGCTTCTATTGCCAATTTGACGGCCTCT12.0No Hit
GGCGGGTCGCGGGGGCGCCGCGCGGCCAGGGGCGGGGCCGGGGGGGGGCG12.0No Hit
TGTTCTTCTCCTCCAAAGCCAGGTCGGGGTTTATGTAGGCCAGCCGCTCT12.0No Hit
TGACGCTGAAGTGCGAAAGCCGGGGGAGCGAACAGGATTAGATACCCTGG12.0No Hit
CAACTTTTGAGCCTTTGCCTTTAAAATTGACTTCCAGCCCTTGCCATTCA12.0No Hit
CTCTGGGGAAAAAAGTAGTAAAAAGCTAAATGCAATCAATCAGCAATTGA12.0No Hit
GCCACATTGAAACAGAGAGAAAAGGTTTGGAAAATCAGGTGCTGAATTTA12.0No Hit
CGCTCACGAGTTCGGTCCTCCACACAATTTTACCTGTGCTTCAACCTGGC12.0No Hit
TCGACCCTCACCCCATCCAGGTACACAAGCCCGAGGTGGGCCTCCAGCTG12.0No Hit
GCCCCGGCCGTCCTGTCGCCTGTCGAAAGGCCCCGATCGTCGGGGTCAAT12.0No Hit
CTACATTTAAGGTTGGTAAGGATGACTTGGAGGTCCATGGTTTTCATTAC12.0No Hit
CGAACCCACCAGGGTTGAAAACCTGGGGGATCAACTGTGGCTAGGGGTGA12.0No Hit
GGAGGTGGGTGATGCGCATGTCAGTGCTACCTCATGCCACCTCCTCTCTG12.0No Hit
GGGCGGGCTTAGCACATGGAAGTCGAGGGGTAGGATATCGCAGCGTGCTT12.0No Hit
GGTTGATCTGCGCACAGTGTTCTTGGCCAGCTGGTGAACTTTCCCTGCAC12.0No Hit
TGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAAAG12.0No Hit
GAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGG12.0No Hit
ATAGTAGATTCTTCATCTACTGGGTTTCCCCATTCGGACATCTTGTATTA12.0No Hit
TTACTCGCATCAGGAGTATCAATCACCTGAGCTAACCATAGTCTAATAGA12.0No Hit
CCTTAAGTAGGGACCTGTATGAATAGCTCCGCGAGGGTTCAGCTGTCTCT12.0No Hit
AATACCACTACTCCTATTGTTTTTTTACTTATTCAATGAAGCGGCGCTGG12.0No Hit
CAGTGCTTCTCTTTCTCACTGCTTCCAATGATGAAACAGCACCTAGGACA12.0No Hit
AGGAGATCTCGGAGCCGATGGCCGGGGTGGCATCGTCCTTGACCGTCTTC12.0No Hit
CTACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGG12.0No Hit
GGGCAGCATGGTGAGATCCCATCTGTACAAAAAATAATTTGAAAAAATTA12.0No Hit
TGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATT12.0No Hit
CTTCCTGGGGACAATGTGGGCTTCAATGTCAAGAATGTGTCTGTCAAGGA12.0No Hit
GCGGCAAGTTCTCATTGTTGGGATGCATTTTAGTGTACGAAATAAACTGG12.0No Hit
CAGCAATATGCCACTGTACAAAACTGAAGAAAAGTTCCTAATTTGTACTT12.0No Hit
GGAGAACTGAACCATCTAAGTACCCGGAGGAAAAGAAATCAACCGAGATT12.0No Hit
TTAAGCACGAATGTCCTAAGGCATCCATTGCAACTAATAGGTTAGCATCT12.0No Hit
GTAGCTTTTATCCGTTGAGCGACGACCCTTCCATTCGGAATCGCCGGATC12.0No Hit
GGGTGGAGCTTCATGAAAAGCAAATGTAGGTATAAGTAACGATAAAGAGT12.0No Hit
CTGGATTTTTGAGTGTTACCTCCCACTTAGGAGCAGAGTGAGCGTAGACA12.0No Hit
CGCGCCCGACGACTTCGATCACGTCAACGACCGCCCGGGCCACGACCTGC12.0No Hit
GCGGTGGTGTGTGCGTAGTAGGCATTGATGTTGGCGAGCAGGGTGCTCAT12.0No Hit
CTTGTACTGAACTGATAGGTGGCCTAGTGGTTATGCCCTGTACTACCATT12.0No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGA50.0145.08
GCTAGAC50.0145.03
AGCTAGA50.0145.02
GAGCTAG50.0145.01
CGGAAGT50.0145.0145
CTGCGAG50.0145.09
GACTGCG50.0145.07
CTAGACT50.0145.04
AGACTGC50.0145.06
TAGACTG50.0145.05
Produced by FastQC (version 0.11.5)