FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_4329.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_4329.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15599940
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA35100.025.8142664
ATTCGTT73750.025.261147145
TGCCGTG95850.023.445068145
AACGTAT171450.022.5767826
TGTCCTA85100.022.316635
GAACGTA204550.019.1005635
CTATTAG68800.019.0697778
ACGTATT213500.018.8090727
CGGGAAC187050.018.6011162
GTATTCA218300.018.3290869
GGAACGT189100.018.2461954
CGTATTC221650.018.0847648
CTACTCT117250.017.557439
TATTCAC204500.017.510129
CGTATGG52900.017.129168145
CCGGGAA207750.016.4412351
GAGTCTA53600.016.228262
TGCCGTA127950.015.9192119
CGTCTTG108950.015.103602145
TCGTATA73050.014.984377145
Produced by FastQC (version 0.11.5)