FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_4329.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_4329.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15599940
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA35300.025.8733544
ATTCGTT73400.024.986395145
TGCCGTG95700.023.178677145
AACGTAT172250.022.4719246
TGTCCTA84750.022.0666735
CTATTAG68600.019.3367048
GAACGTA205250.019.0354215
ACGTATT213950.018.7695127
CGGGAAC187200.018.586212
GTATTCA220000.018.1874529
GGAACGT190400.018.1596854
CGTATTC222850.017.9873818
TATTCAC206000.017.6289319
CTACTCT116350.017.3817429
CGTATGG53100.016.518476145
CCGGGAA208300.016.3979821
GAGTCTA53650.015.9429162
TGCCGTA128450.015.6315169
TCGTATA73150.015.360187145
CGTCTTG109300.014.789853145
Produced by FastQC (version 0.11.5)