FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30981.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30981.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15495416
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGA34600.016.1308254
CCAACCG36750.015.7788213
ACGTCTT39350.015.29005145
ACACGTA51050.015.051704145
AGCTCGC58600.014.22466859
GAGCTCG63100.013.210238
TCCGGAC22650.012.481648145
CGAGGGA64600.012.00585658
AACCGAG46500.012.002725
GAGCACC130950.011.957043145
GAGAATG349250.011.893064145
ATCCCGA67850.011.8580984
CGTGCGT20500.011.668158
TCGTGCG21550.011.4359857
TCGGAAT61850.011.368615145
AGTCACG33550.011.235343145
AGGCGTA24900.011.061818
TGCGCGA28400.010.9746849
ACCGAGG53350.010.8692326
TTACGAC12801.09139364E-1010.7593385
Produced by FastQC (version 0.11.5)