FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30981.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30981.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15495416
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGA34600.016.3403974
CCAACCG37000.015.8681913
ACGTCTT39500.015.048495145
ACACGTA51000.014.497941145
AGCTCGC58600.014.4721269
GAGCTCG63350.013.3870018
TCCGGAC22900.012.978505145
AACCGAG46350.012.1980095
CGAGGGA64650.011.8845148
ATCCCGA68500.011.8514524
GAGCACC131250.011.819273145
GAGAATG350350.011.710909145
TCGGAAT62500.011.5983515145
AGTCACG33150.011.152262145
TCGCGGT49550.011.118502145
CGTGCGT20250.011.0963658
TTACGAC13151.4551915E-1111.0242315
TGCGCGA28350.010.9940949
CGCGTAT23150.010.959565145
AGGCGTA25300.010.8869728
Produced by FastQC (version 0.11.5)