FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30981.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30981.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13940757
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT292720.20997425032227446TruSeq Adapter, Index 7 (97% over 36bp)
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC244010.17503353655759152No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG181350.1300861925934151No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT168160.1206247264764747No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT139470.10004478236009709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG183350.033.76467145
GATCCTG43450.028.5274055
AACGTAT268300.028.3947476
GGCCAAC61700.027.856791
GAACGTA291900.026.2480415
TCCCTGA80200.026.1203783
TCAGATT82450.026.0228862
GTATTCA299500.025.8482079
ACGTATT300100.025.6515087
CCGGGAA274300.025.4341451
GATCATG45450.025.3582445
CGTATTC306950.025.1735238
GGAACGT301900.024.562274
GGCTATT31800.024.39171145
GAGTCTA38350.024.0045052
CCCTGAG87750.023.955594
GGTTAGA84900.023.3936944
TTGAACG92650.022.9232717
CTCCCTG92750.022.7422522
GAAACTA65850.022.676054
Produced by FastQC (version 0.11.5)