FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30981.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30981.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13940757
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT292720.20997425032227446TruSeq Adapter, Index 7 (97% over 36bp)
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC244010.17503353655759152No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG181350.1300861925934151No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT168160.1206247264764747No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT139470.10004478236009709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG183650.033.55184145
GATCCTG42950.028.6909225
AACGTAT268200.028.2164296
GGCCAAC61800.027.4594481
GAACGTA291350.026.1236025
TCCCTGA79700.026.1025143
TCAGATT82700.026.0320382
GTATTCA299300.025.6962079
ACGTATT300100.025.4826897
GATCATG45100.025.394485
CCGGGAA274750.025.31311
CGTATTC306400.025.0770178
GGAACGT301650.024.4145724
GGCTATT31300.024.318304145
GAGTCTA38350.024.1936722
CCCTGAG87450.023.8721454
GGTTAGA84950.023.6360654
TTGAACG92650.023.0017387
CTCCCTG92250.022.7085112
GAAACTA65300.022.5341724
Produced by FastQC (version 0.11.5)