FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30978.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30978.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13796210
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT269810.19556820315144524No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT181480.1315433731437837No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC157010.11380661790448246No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA153220.1110594866271244No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC138980.10073781132644401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG165550.034.7667145
ATCCCGA159200.028.8206984
GATCCCG161650.028.2046263
CGTCTTG83450.026.05956145
GCGATAT30800.024.476809145
TGCCGTG72250.023.577742145
GTCACAC95150.022.778996145
TCCCGAG212050.021.8768885
ACGAGCA38900.021.0558454
ACACGTA47650.020.99367145
CCGAGGC262100.020.32657
CGAGCAA44900.020.0179315
GAGCACC115550.019.949377145
TACGGAC110800.019.8219645
CGAGGCC243350.019.6289168
CGGATGT25050.019.1071411
AACGTAT102100.018.8133246
GGGATCC255850.018.7075981
CCCGAGG266100.018.6046376
CGTGCGT32800.018.562988
Produced by FastQC (version 0.11.5)