FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30978.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30978.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13796210
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT269810.19556820315144524No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT181480.1315433731437837No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC157010.11380661790448246No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA153220.1110594866271244No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC138980.10073781132644401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG166350.034.817223145
ATCCCGA159100.029.1575894
GATCCCG161850.028.4831373
CGTCTTG84150.025.928806145
GCGATAT31100.024.706753145
TGCCGTG71950.023.373695145
GTCACAC95500.022.695408145
TCCCGAG212000.022.121285
ACACGTA48550.021.201632145
ACGAGCA38650.021.0044044
CCGAGGC262000.020.5554897
GAGCACC115600.020.379602145
CGAGCAA45000.019.9733545
TACGGAC111100.019.8988325
CGGATGT25150.019.8962751
CGAGGCC243850.019.7966528
GGGATCC255300.018.9751991
CGTGCGT32650.018.8701788
CCCGAGG266200.018.7337116
GGATCCC255750.018.7056712
Produced by FastQC (version 0.11.5)