FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30908.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30908.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072756
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC276370.1833573103684555No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG236370.15681936335995886No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT185340.12296357746386925TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA44650.050.490654
GATCCTG105700.040.1191255
TCGATCT56050.039.9628373
CTATTAG85000.035.476618
CTCGATC63300.034.4694862
CTGGCTC134600.031.0740919
TATTAGT107400.029.292289
TGTCCTA62600.029.0649765
CCGGGAA340450.028.8195041
ATCTATT97000.027.2022346
TGATCCT141950.026.5546134
AACGTAT398050.025.386346
GGGAACG417800.024.3423613
CAAGTTA74150.024.244385
GGAACGT354400.024.1970844
GTGTCCT76700.023.910974
TGGGCTA154450.023.7484152
GTATTCA438150.023.178389
TCCTGGC181800.023.0861997
CGTATTC440600.023.0659458
Produced by FastQC (version 0.11.5)