FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30908.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30908.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072756
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC276370.1833573103684555No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG236370.15681936335995886No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT185340.12296357746386925TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA44350.050.015264
GATCCTG106000.040.2109875
TCGATCT56050.039.1871033
CTATTAG84000.035.4674578
CTCGATC63100.034.1194342
CTGGCTC134700.031.3200869
TATTAGT106450.029.1451039
TGTCCTA63150.028.9268045
CCGGGAA339150.028.6522791
ATCTATT96500.027.042876
TGATCCT142500.026.6048954
AACGTAT397050.025.5234586
CAAGTTA74350.024.5693025
GGGAACG416950.024.2704373
GGAACGT352950.024.235034
TGGGCTA154250.024.155312
GTGTCCT77250.023.8347134
GTATTCA437450.023.265179
TCCTGGC181750.023.2121877
CGTATTC439100.023.1942528
Produced by FastQC (version 0.11.5)