FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30907.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30907.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308158
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC175060.1422308683395192No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG154160.12525026084325536No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT151690.12324346177551508TruSeq Adapter, Index 19 (97% over 38bp)
CGAGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCC124460.10111992387488039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA76150.041.218365
CGTCTTG61200.039.445164145
AGCTATG129300.036.499207145
GTGTCCT106150.030.047424
CTACTCT112450.029.1373429
CGATCTA32500.027.6574944
TGCCGTG72300.027.573719145
ATCTATT36100.026.7065126
GATCCTG103000.026.5324215
CCTACTC125750.026.516788
GTCCTAC120550.026.2776786
GGAACGT198000.026.0668954
AACGTAT315100.025.7657246
CGGTGTC118600.025.6098692
TGGGCTA81450.025.2758222
GTATTCA333100.024.3950639
CGTATTC345400.023.5892968
CCGGGAA228900.023.2239251
TATTCAC349600.022.4557749
GGCTACA94950.022.4453754
Produced by FastQC (version 0.11.5)