FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30907.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30907.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308158
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC175060.1422308683395192No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG154160.12525026084325536No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT151690.12324346177551508TruSeq Adapter, Index 19 (97% over 38bp)
CGAGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCC124460.10111992387488039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA76350.041.0152175
CGTCTTG61300.039.144234145
AGCTATG128300.036.61412145
GTGTCCT105900.030.0497324
CTACTCT112450.029.0728349
TGCCGTG71850.027.645475145
CGATCTA32050.027.1409654
GATCCTG103350.026.6528365
CCTACTC125950.026.4746348
GTCCTAC121350.026.1042946
ATCTATT35900.026.0474686
GGAACGT198900.025.9488014
AACGTAT315400.025.741076
CGGTGTC118400.025.4080852
TGGGCTA81200.024.9964052
GTATTCA333700.024.3511629
CGTATTC345300.023.5960888
CCGGGAA229350.023.1464181
TATTCAC349500.022.6073479
GGCTACA94900.022.227924
Produced by FastQC (version 0.11.5)