FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30905.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30905.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9803480
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC217310.22166618384492037No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG164070.1673589378465606No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA132010.13465626491817193No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC106960.10910411404929678No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT106130.10825747591671529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA21000.043.4926224
GATCATG39300.039.6561245
GATCCTG51450.037.758425
TTGATCA42150.035.7707373
TCAGATT67600.035.2791442
TTTGATC96500.035.0799032
TGTCCTA42950.034.2607775
CAGATTG71300.033.752873
TCGATCT26000.033.4556923
CCGGGAA192350.031.8944661
TTGAACG76950.030.991837
CTATTAG41450.030.0791858
CTGGCTC64550.029.421819
GCGCATG57100.028.946222145
AACGTAT262400.028.6193856
TATTAGT48450.028.4265529
ATGGCTC53500.028.0466469
TGATCCT67450.027.6194824
CGAGCAA37300.027.5958585
CAATTAG47600.027.5636049
Produced by FastQC (version 0.11.5)