FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30905.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30905.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9803480
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC217310.22166618384492037No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG164070.1673589378465606No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA132010.13465626491817193No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC106960.10910411404929678No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT106130.10825747591671529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA21050.043.7336044
GATCATG39050.039.3530545
GATCCTG51600.037.648595
TCAGATT67750.035.5219542
TTGATCA41900.035.4651033
TTTGATC96450.034.9477122
TCGATCT25550.034.612293
CAGATTG71250.034.081713
TGTCCTA43250.033.8554735
CCGGGAA192550.031.6358031
TTGAACG76200.031.5821957
CTATTAG41800.030.1741058
CTGGCTC64800.029.3082529
GCGCATG57400.029.047474145
TATTAGT48500.028.6961179
AACGTAT261900.028.590946
ATGGCTC53150.027.822149
TGATCCT67450.027.7269064
TTGATCC69950.027.1503223
TGGGCTA65800.027.1004982
Produced by FastQC (version 0.11.5)