FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30905.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30905.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14653360
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATTA48800.037.8772858
CTATTAG61950.036.3896268
TTCTGGC74950.028.1436147
CGAGCTA42150.027.5157224
CAATTAG70300.026.2932859
TTTGATC88050.024.0389212
TTGATTC83000.023.754773
TATTAGT101900.023.4746869
GATCCTG100850.023.1439235
AGCAATT80350.022.012157
CGATCTA14750.021.1317254
TCTGGCT109850.019.8620918
ATTCTGG105150.019.6469026
CTGGCTC213550.018.8048639
CGACCTA20550.018.342144
TCGAGCT68900.017.3590033
CGAGCAA110200.016.970525
TTGATCC129750.016.9275953
GAGCAAT106150.016.798626
TCACGAG106050.016.609522
Produced by FastQC (version 0.11.5)