FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30904.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30904.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016551
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT387740.27663010679303346No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC269550.19230836458983383No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT263990.18834162555396117No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC193550.13808675186927227No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG173030.12344691643472064No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA171210.12214845149851772No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT170370.121549159989501No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG140210.10003174104670971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA45600.039.423256145
GAGTCTA48500.036.0186162
AACGTAT240350.033.113876
TGCCGTG83900.031.448874145
AAATCTG54450.031.018705145
GATCCTG42100.030.9913545
GAACGTA258000.030.9326955
CGAAATG142250.030.880617145
TCGATCT23050.030.817953
GAAACTA110650.029.7409324
CGGGAAC263800.029.6482032
ACGTATT277300.028.8058247
GTATTCA279700.028.7916879
TACTTCC55350.028.6798345
CGTATGG42050.028.443537145
CGTATTC283550.028.3498338
TTACTTC53800.028.2935314
GGAACGT281950.027.5595974
TCTAACA65550.026.6498345
AAGAAAC122950.026.5299022