FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30903.01.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30903.01.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15803871
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA67150.032.3845525
CTATTAG57250.031.1474348
CTGGCTC165750.025.1027349
TATTAGT85350.023.7802939
ACATTAA63200.023.05441145
GATCCTG204700.022.4863285
TGATCCT204700.021.9196034
CTACTCT103650.020.4908989
GTGTCCT108300.020.414154
AACGTAT274150.019.8572856
CGATCTA59800.019.2732894
TTTGATC220150.019.261812
TCCTGGC219750.018.967197
CCTACTC119700.018.530628
ATCTATT72000.018.2227656
GGAACGT212450.018.2198354
CCTGGCT218850.018.1177168
TTGATCC232200.017.2944243
CCGGGAA246900.016.9181291
CGTATTC323000.016.8538888
Produced by FastQC (version 0.11.5)