FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30900.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30900.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17335039
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA211070.12175917227529745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCTC190200.020.617889
GATCCTG255100.020.1461965
CATTATG96200.018.53723145
TGATCCT275950.018.0723784
GTTTGAT267650.016.9063571
AGTACTA88450.016.8821078
CGATCTA25200.016.3957614
TAGTACT92050.016.3793547
CAATTAG91450.016.3282933
ATTAGTA117650.014.8484935
CCTGGCT264250.014.3738658
AATTAGT110250.013.9384514
GCGATAA212750.013.6284883
TCCTGGC283050.013.6240357
TTGATCC301750.013.3322353
CGATAAG214900.013.3234884
TTTGATC317950.013.1317372
CTATTAG59850.012.595828
ATAAGCC235550.012.4631886
ACACGTA52300.012.335966145
Produced by FastQC (version 0.11.5)