FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30900.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30900.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17335039
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA211070.12175917227529745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCTC190400.020.710539
GATCCTG256750.020.270975
CATTATG96450.018.489214145
TGATCCT277300.018.1936534
GTTTGAT267850.017.0834711
AGTACTA88450.016.9641368
TAGTACT92600.016.3604267
CAATTAG91500.016.3194453
CGATCTA25600.015.8565454
ATTAGTA118200.015.0248145
CCTGGCT263900.014.4479298
AATTAGT110550.014.031874
TCCTGGC284000.013.7316667
GCGATAA213100.013.7082133
TTGATCC301950.013.4675043
CGATAAG215600.013.3811934
TTTGATC317750.013.2541262
ACACGTA52450.012.577127145
ATAAGCC236300.012.5157536
CTATTAG59600.012.4054698
Produced by FastQC (version 0.11.5)