FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30900.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30900.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18014631
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC481130.26707735506766694No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG341360.1894904203144655No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA205760.11421827069341582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA70900.044.369025
CGATCTA29150.044.0118754
TCGATCT28350.043.9754753
ACGAGCA81100.042.98924
GATCATG42500.042.2959825
CCGGGAA440000.040.28161
GAGCAAT91500.039.6083226
CGTCTTG103850.039.09004145
GATCCTG60400.037.561575
CTATTAG62600.036.820258
AGCAATT98750.036.7000667
GTGTCCT91900.034.9400374
CAATTAG120900.034.5930029
GCAATTA123450.034.0542148
AACGTAT613250.032.9764756
GGGAACG579750.032.5438233
TTTGATC124900.032.324482
CTCGATC39650.032.1742362
CGAGCAA122550.031.0514815
GTATTCA659500.030.8178259
Produced by FastQC (version 0.11.5)