FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30900.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30900.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18014631
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC481130.26707735506766694No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG341360.1894904203144655No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA205760.11421827069341582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT28400.044.153433
TGTCCTA70400.044.0664255
CGATCTA29050.043.9139024
ACGAGCA80700.042.663414
GATCATG43050.041.755655
CCGGGAA441500.040.3751871
GAGCAAT91450.039.2337156
CGTCTTG104100.038.36947145
GATCCTG60750.037.822455
AGCAATT98800.036.3147057
CTATTAG62100.035.9495478
GTGTCCT91100.034.769514
CAATTAG120550.034.2725759
GCAATTA123800.033.6066748
AACGTAT612200.033.1040956
GGGAACG578550.032.736723
TTTGATC124950.032.5436972
CTCGATC40350.032.1550982
CGAGCAA121300.031.0727185
GTATTCA658950.030.9535689
Produced by FastQC (version 0.11.5)