FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30893.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30893.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15805174
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA36500.024.6251874
CTATTAG78950.022.8609288
CCGGGAA159150.022.144921
CGAGCTA35600.020.5646864
CGGGAAC178200.019.9725152
GATCCTG121300.019.4808335
GGAACGT178650.019.4755154
AACGTAT208650.019.1418516
GGGAACG192800.018.27183
TGCCGTG74050.016.641657145
GAACGTA264750.015.63330655
CGAAATG166100.015.580137145
TCGATCT57600.015.1011793
GTATTCA276050.014.8619329
CGTATTC276450.014.8404288
TATTAGT128550.014.660479
TTGATCC152850.014.41651253
TTTGATC177150.014.3214122
CTGGCTC159650.014.02930459
GATCTAT71650.013.8597395
Produced by FastQC (version 0.11.5)