FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30891.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30891.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14559836
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA23800.040.202184
TGTCCTA58750.037.754285
GATCCTG93650.037.0749325
GCAATTA87250.033.7292948
CTGGCTC108600.032.7049069
CAATTAG89200.032.6668979
AGCAATT73950.032.346257
GAGCAAT79800.032.246176
TGATCCT108650.031.3560094
TTTGATC133450.030.9065692
GTGTCCT75500.029.858374
CATTATG110200.028.350372145
CTATTAG65450.027.5763248
ACGAGCA80600.027.4294724
TCACGAG86250.026.7254562
TTGATCC128850.026.496813
GGCTCAG107250.026.0205254
CGAGCAA95650.025.917545
TCCTGGC134250.025.1065377
GCTCAGA73200.024.6570385
Produced by FastQC (version 0.11.5)