FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30891.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30891.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14559836
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA23850.040.725854
TGTCCTA59250.037.802795
GATCCTG93050.037.158295
GCAATTA87100.033.5378078
CTGGCTC107950.032.7004789
CAATTAG89350.032.449899
GAGCAAT79150.032.236326
AGCAATT73400.032.1936957
TGATCCT108250.031.4719544
TTTGATC133550.030.7206752
GTGTCCT75750.030.0469724
CATTATG110350.028.246218145
CTATTAG65700.027.3611358
ACGAGCA80100.027.0577984
TCACGAG85700.026.3895532
TTGATCC129150.026.266953
GGCTCAG107150.025.9095784
CGAGCAA94750.025.857785
TCCTGGC133300.025.068027
GCTCAGA73300.024.7223515
Produced by FastQC (version 0.11.5)