FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30830.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30830.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12981437
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT299780.2309297499190575TruSeq Adapter, Index 3 (97% over 36bp)
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC233400.17979519524687443No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG228260.17583569523158338No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC172720.13305152580565618No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG149290.11500267651416403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG63800.046.581935
CGATCTA37500.043.4914474
TCGATCT37300.042.7528273
GCGCATG92700.031.904829145
CTCGATC50700.031.739322
CTATTAG95800.031.0978668
CCGGGAA272900.029.5509471
TGCCGTG82900.027.719206145
TGATCCT108000.027.3163874
CTGGCTC113750.027.0825849
ATCTATT66550.026.9031526
AACGTAT337950.026.6822366
TCGAGCT36000.025.3699113
TTTGATC125150.025.2527242
GGAACGT329350.025.1339664
GCATCTG68000.024.625162145
GTTAGAG104800.024.2772855
GTATTCA374850.024.268279
GAACGTA378750.023.9801945
CGGGAAC343900.023.712262
Produced by FastQC (version 0.11.5)