FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30830.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30830.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12981437
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT299780.2309297499190575TruSeq Adapter, Index 3 (97% over 36bp)
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC233400.17979519524687443No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG228260.17583569523158338No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC172720.13305152580565618No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG149290.11500267651416403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG63400.046.647215
CGATCTA37450.043.5495574
TCGATCT37300.042.7528653
GCGCATG92050.031.893906145
CTCGATC50650.031.7706872
CTATTAG96550.031.3067878
CCGGGAA272100.029.4241851
TGCCGTG82550.027.836761145
TGATCCT108350.027.2281764
CTGGCTC113200.027.0861349
ATCTATT66950.026.7424456
AACGTAT337600.026.5810996
TCGAGCT36000.025.9739843
TTTGATC125450.025.2501452
GGAACGT328650.025.0110824
GTTAGAG104550.024.5433585
GCATCTG68350.024.499088145
GTATTCA375000.024.161949
GAACGTA378550.023.8785
CGTATGG54050.023.604425145
Produced by FastQC (version 0.11.5)