FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30786.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30786.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14991560
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATCC319330.2130065183343161No Hit
CCGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATC269870.180014621560398No Hit
CACACGTGTTACAATGGGGGGTACAGAAGGCCGCTACCCGGCGACGGGAT173360.11563839920595322No Hit
CAGAAGGCCGCTACCCGGCGACGGGATGCTAATCTTTAAAACCTCTCTCA152160.10149710904001985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG92450.047.3577275
TGATCCT91250.046.7095074
CTGGCTC111350.038.3432439
CCTGGCT118850.036.0453388
TTTGATC142050.035.771782
TCCTGGC124600.034.847347
TTGATCC143200.030.6247963
ATCCTGG153150.029.7711726
TATGATG153450.029.242533145
CGATCTA19700.029.0684384
CCGGGAA391500.026.6743851
AACGTAT506800.023.9287595
AAATCGA106850.023.5405064
GTATTCA542800.022.3417388
GAACGTA556450.022.2105374
TATTCAC556250.021.9059169
CGTATTC553600.021.9058787
CGGGAAC497200.021.79131
ACGTATT564200.021.789896
CGGAGTA53650.021.4826684
Produced by FastQC (version 0.11.5)