FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30786.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30786.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14991560
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATCC319330.2130065183343161No Hit
CCGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATC269870.180014621560398No Hit
CACACGTGTTACAATGGGGGGTACAGAAGGCCGCTACCCGGCGACGGGAT173360.11563839920595322No Hit
CAGAAGGCCGCTACCCGGCGACGGGATGCTAATCTTTAAAACCTCTCTCA152160.10149710904001985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG92850.047.0756075
TGATCCT91500.046.5026364
CTGGCTC111850.038.2366189
CCTGGCT118950.035.9540758
TTTGATC141650.035.8215942
TCCTGGC124700.034.819377
TTGATCC143500.030.5607533
ATCCTGG154050.029.6442766
TATGATG152850.028.883036145
CGATCTA19500.028.2513734
CCGGGAA391350.026.7215651
AACGTAT507550.024.0504865
AAATCGA106400.023.7081784
GTATTCA543250.022.4566488
GAACGTA557350.022.291714
CGTATTC553650.022.0479077
TATTCAC556800.022.0274689
CGGGAAC497350.021.9450381
ACGTATT564250.021.9164146
CGGAGTA53450.021.563044
Produced by FastQC (version 0.11.5)