FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30783.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30783.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11093977
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC454970.41010541125152866No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG338910.30549008709861214No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA179470.16177246446427643No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA158380.1427621492274592No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC138730.12504983559998367No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT135440.12208426247864045No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT131100.11817222985048555No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGATAGTGCGAGC127000.11447653082388759No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC118010.10637303466556672No Hit
CACACGTGCTACAATGGCGTATACAGAGGGAAGCGATAGTGCGAGCTGGA116490.10500292185570603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA229450.040.01221
GGTTAGA88550.036.508284
TGTCCTA53550.034.381225
GAGCAAT81400.033.8379756
AACGTAT301550.033.1474466
GCAATTA93500.032.8698278
GGGAACG302400.032.1673933
TCACGAG75800.031.9390812
ACGAGCA80500.031.3349154
CAATTAG98950.031.2057789
ACGTATT334500.030.2938337
GAACGTA341450.029.5501175
AGCAATT94000.029.5334457
GGAACGT333550.029.2284974
GTATTCA352500.028.7056589
TCTCGTT58600.027.710007145
CGAGCAA99750.027.1046035
CCATTGT225750.026.0423145
GTGTCCT73350.025.5943894
TATTCAC321000.025.4709579