FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n01_MT_30783.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n01_MT_30783.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11093977
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC454970.41010541125152866No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG338910.30549008709861214No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA179470.16177246446427643No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA158380.1427621492274592No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC138730.12504983559998367No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT135440.12208426247864045No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT131100.11817222985048555No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGATAGTGCGAGC127000.11447653082388759No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC118010.10637303466556672No Hit
CACACGTGCTACAATGGCGTATACAGAGGGAAGCGATAGTGCGAGCTGGA116490.10500292185570603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA230400.039.9106031
GGTTAGA88400.036.160324
TGTCCTA53800.034.490835
GAGCAAT81200.033.9212276
AACGTAT301950.033.1514556
GCAATTA93300.032.94028
GGGAACG303250.032.1489943
TCACGAG75950.031.876062
ACGAGCA80450.031.5346434
CAATTAG98800.031.2530739
ACGTATT334900.030.3225027
AGCAATT93900.029.5648177
GAACGTA342250.029.5233255
GGAACGT334550.029.2061844
GTATTCA352600.028.7591139
TCTCGTT58450.027.533125145
CGAGCAA100200.027.0551415
CCATTGT226150.025.964231145
GTGTCCT73700.025.6695924
TATTCAC321100.025.395249