FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25844259
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG922310.356872294152446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA248650.055.0441862
GATCGGA246000.055.0346641
TCGGAAG245950.054.256263
ACACGTC209150.054.2453313
CGGAAGA246300.053.5533454
CGTCTGA207550.052.9085216
ACGTCTG210250.052.34568815
GCACACG221950.052.17437411
CACACGT227300.051.0378212
CACGTCT219650.050.83938614
AGAGCAC306800.040.3112228
AGCACAC306000.039.2386510
GAACTCC274950.038.5262421
CTGAACT295900.036.7445919
GAAGAGC374050.034.2438856
TGAACTC322450.033.5454620
GAGCACA367800.033.2451639
TCTGAAC336650.032.71267718
AACTCCA323650.032.51296622
GTCTGAA348850.031.87955917
Produced by FastQC (version 0.11.5)