FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41933034
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1511750.36051529207259364TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA204550.055.79794741
CTCGTAT206750.055.7288642
TCGTATG210500.055.41770643
ATGCCGT209450.055.2107247
CGGCTAC219700.055.10500333
ACGGCTA223750.054.57736632
CGTATGC215500.054.4240644
TGCCGTC210700.054.1688448
GCCGTCT207800.053.51050649
TATGCCG218500.053.46851746
CCGTCTT212700.052.5739750
TCACGGC235550.052.06579630
ATCTCGT222500.051.2807640
CGTCTTC211900.051.1702351
GTCACGG241750.050.81755429
CATCTCG225950.050.59070639
CGTCTGA249350.050.30606516
CACGGCT250200.048.989531
AGTCACG251700.048.94761328
ACGTCTG261900.047.92228715
Produced by FastQC (version 0.11.5)