FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44559285
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC504300.11317506553347972TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA40350.058.2898541
TATCGCG86600.058.1582239
CGCGTAT40450.057.1938942
ATCGCGT61350.055.68041240
CTTATCG181250.054.86070637
TCTCGTA97750.050.5216441
TTATCGG93650.050.26696438
CTCGTAT97000.050.26273342
TTATCGC103450.050.24167638
CGTCTGA400300.050.12560716
TATCGGG91900.049.92928739
CGTATGC108700.049.52113744
TATCTCG137750.049.1397639
TCGTATG90500.048.53565243
ACGTCTG413450.048.51467515
ACACGTC420700.047.84516513
GCTTATC401950.047.46465736
TATGCCG126550.047.0996646
ATGCCGT104850.046.76656347
CACGTCT440300.045.73907514
Produced by FastQC (version 0.11.5)