FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41211869
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1414520.3432312181716389TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA34350.060.32052241
CTCGTAT197400.059.308842
TCTCGTA199000.059.0605841
TCGTATG194500.059.05933843
CGTATGC214700.058.0837244
CGTCTGA404700.057.7963616
CGCGTAT36200.057.23784342
TATGCCG218450.057.23041546
ATGCCGT202700.056.70442247
ACACGTC416800.056.36227813
CATCGCG63000.056.27818339
ACGTCTG415900.056.24834415
ATCGCGT49500.055.78821640
TGCCGTC200650.055.46972348
ATCTCGT231250.055.4554740
CATCTCG247650.054.5814639
GCCGTCT185050.054.43393349
CCGTCTT197100.053.8939650
CACGTCT438650.053.4747214
GCACACG466150.050.5463911
Produced by FastQC (version 0.11.5)