FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45135525
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15254033.3796061971141356TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1735350.065.8193642
TCTCGTA1710550.065.71387541
TCGTATG1810350.065.7004243
CACGATC1697750.065.5619136
ACGATCT1695600.065.5423437
CGTATGC1820450.065.52795444
CGATCTC1698850.065.4396238
GATCTCG1700800.065.3874439
TCACGAT1776500.065.3173535
TATGCCG1823300.065.3061646
ATGCCGT1818650.065.2710647
ATCACGA1813550.065.2356234
ATCTCGT1708550.065.1495340
CCGTCTT1793700.064.80556550
CGTCTGA1888300.064.7741416
TGCCGTC1823050.064.7200948
CATCACG1856100.064.6606333
GCCGTCT1796650.064.6173349
ACACGTC1901550.064.554713
CGTCTTC1780650.064.482251
Produced by FastQC (version 0.11.5)