FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42183687
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC8460152.005550154968673TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC1970570.46714029525204853TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA987650.066.5701441
TCGTATG1190200.066.456943
CTCGTAT985600.066.4244342
CGTATGC1196850.066.1339744
AATCTCG1057100.066.0603239
ATGCCGT1117500.065.9935847
CGTCTGA1608850.065.9787516
TGCCGTC1096400.065.73765648
TATGCCG1192500.065.6672546
GCCGTCT1021100.065.5231749
ATCTCGT1050300.065.4584640
ACGTCTG1623650.065.420615
CCGTCTT1057500.065.39262450
ACACGTC1629050.065.356613
CGTCTTC987450.064.6084851
CACGTCT1647850.064.5853214
GTCGTAT274950.063.7247242
GCACACG1675250.063.6638911
CACACGT1679500.063.4747912
GTATGCC1258750.063.4680245
Produced by FastQC (version 0.11.5)