FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42453981
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1331500.313633720239334TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA54950.064.84093541
CGCGTAT56400.063.36002342
ATCGCGT88700.063.33169640
CATCGCG128300.062.66182739
CTCGTAT192900.061.87153242
TCTCGTA194150.061.81577341
CGTCTGA595450.060.92381716
TCGTATG188150.060.64312443
CGTATGC209700.060.30276544
ACGTCTG608850.059.67472515
ACACGTC612450.059.3589413
ATGCCGT190150.058.9003347
ATCATCG218850.058.83726537
ATCTCGT253950.058.40936340
TATGCCG225750.058.27874846
CATCTCG283850.057.3243839
CACGTCT634950.057.27150314
TGCCGTC199600.055.58566748
GATCATC596350.054.7229836
TCATCGC151800.054.71333338
Produced by FastQC (version 0.11.5)