FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42063587
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC840720.19986883191868537TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA125000.051.21224641
CTCGTAT125250.051.02607342
TCGTATG125500.050.9520143
CGTATGC127800.050.19935244
CGCCAAT135600.049.8419833
TATCTCG134600.048.2879239
ATGCCGT129800.047.9965847
GTCACGC149500.045.37177729
ACGCCAA150350.045.2784432
TATGCCG144050.044.07481846
ATCTCGT148350.043.8357940
TGCCGTC140600.043.8119248
CCGTCTT138400.043.2945450
CGTCTGA161400.042.78429416
GCCGTCT140550.042.3335449
TCACGCC169000.040.1364830
GATCGGA172500.039.876391
AGTCACG171950.039.75302528
CGTCTTC146250.039.67825751
ATCGGAA175150.039.567172
Produced by FastQC (version 0.11.5)